Addition of Cryoprotectant Significantly Alters the Epididymal Sperm Proteome
نویسندگان
چکیده
منابع مشابه
Corticosterone-Induced Oxidative Stress Alters Epididymal Sperm Fertility in Rats
Activation of the hypothalamic-pituitary-adrenal (HPA) axis releases glucocorticoids, which play an important role in the homeostatic control of the body and the organism’s response to stress (Lin et al. 2004). Corticosterone (CORT) is the predominant circulating glucocorticoid in rodents and its excess levels in the circulation is a major indicator of stress (Lerman et al. 1997, Hardy et al. 2...
متن کاملMaspin alters the carcinoma proteome.
Maspin, a member of the serine protease inhibitor (serpin) family, is a tumor suppressor in breast and prostate cancer. To address molecular mechanisms underlying maspin's activity, we restored its expression in invasive carcinoma cells and analyzed the resulting changes by shotgun proteomics. Using a mass spectrometry-based multidimensional proteomic method, we observed changes to the expressi...
متن کاملCooling of ejaculated and epididymal stallion sperm
After a serious injury or sudden death, epididymis cauda sperm recovery and cryopreservation may present as the last opportunity to obtain genetic material from a valuable stallion. This study evaluated the viability of cooled equine sperm collected by three different methods: sperm of ejaculated (G1), sperm recovered from the epididymal cauda immediately after orchiectomy (G2) and sperm recove...
متن کاملDrosophila sperm proteome evolution
Despite their conserved functional role in sexually reproducing organisms, spermatozoa are a diverse and rapidly evolving cell type. This phenomenon is largely attributed to sexual selection in polygamous species where sperm from multiple males compete to fertilize a limited number of oocytes. Drosophila have proven to be a particularly informative model system for the study of spermatogenesis ...
متن کاملEvaluation of frozen thawed cauda epididymal sperms and in vitro fertilizing potential of bovine sperm collected from the cauda epididymal
In the present study, the fertilizing potential of semen recovered from slaughtered bulls epididymis was evaluated after cryopreservation, by conventional techniques and flow cytometry methods. The cauda epididymal was dissected and sperm were recovered and evaluated for volume, sperm concentration, and membrane and acrosome integrity using a flow cytometer. Sperm fertility potential was tested...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PLOS ONE
سال: 2016
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0152690